The Second National Consultation on the Use of Antiretroviral Pre-exposure Prophylaxis (PrEP) to Prevent HIV Acquisition was held in Kuala Lumpur, Malaysia, on 4 June 2018. The consultation was convened by the Centre of Excellence for Research in AIDS (CERiA) at the University of Malaya, the Malaysian AIDS Council (MAC) and the World Health Organization (WHO) to follow up on recommendations by the first national PrEP consultation held in May 2016.The purpose was to stimulate further dialogue and discussion among various stakeholders involved in the implementation and scale up of PrEP in Malaysia and to learn from the experience of other Asian countries. Participants included members of community-based organizations (CBOs), infectious disease physicians, primary care doctors, researchers, Ministry of Health officials, PrEP users, civil society representatives and international experts.